Journal: Journal of Biological Engineering
Article Title: Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking
doi: 10.1186/s13036-019-0165-4
Figure Lengend Snippet: Evaluation of human, small intestinal monolayer systems. a Schematic and fluorescence side images of the gradient cross-linked scaffold (top) and conventional scaffold overlaying a porous membrane (bottom). Scale bar = 300 μm. b Fluorescence images of the monolayers at day 10 stained for EdU (green), ALP (red), Muc2 (yellow), and nuclei (blue). Scale bar = 100 μm. c Quantification of Edu, ALP, and Muc2 on day 5 and day 10 of culture as a percentage of the Hoechst 33342 (nuclei stain) positive area ( n = 3). Edu, ALP, and Muc2 expression was not statistically different between the two monolayer systems in EM or DM
Article Snippet: The fixed cells were analyzed for the presence of mucin-2 (Muc2) by immunostaining with a rabbit anti-mucin2 primary antibody (α-muc2, 1:200, Santa Cruz Biotechnology, Dallas, TX, #sc-15,334) followed by an Alexa Fluor 488 α-rabbit secondary antibody (1:500, Jackson Immunoresearch, West Grove, PA, # 711–545-152).
Techniques: Fluorescence, Membrane, Staining, Expressing